Flexibacterium Medium

[所属分类:培养基配方] [发布时间:2021-3-1] [发布人:王亚萍] [阅读次数:] [返回]

Flexibacterium Medium

培养基配方

Composition per 1050.0mL:
Tris(hydroxymethyl)aminomethane buffer...................................1.0g
Yeast extract..................................................................................1.0g
CaCl 2 ·2H 2 O ..................................................................................0.1g
KCl................................................................................................0.1g
MgSO 4 ·7H 2 O................................................................................0.1g
Sodium glycerophosphate.............................................................0.1g
NaNO 3 ..........................................................................................0.1g
Cobalamin...................................................................................1.0μg
Glucose solution......................................................................50.0mL
Trace elements.........................................................................10.0mL
pH 7.5 ± 0.2 at 25°C

Glucose Solution:
Composition per 50.0mL:
Glucose .........................................................................................1.0g
Preparation of Glucose Solution: Add glucose to distilled/deionized water and bring volume to 50.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 25°C.

Trace Elements:

Composition per liter:
FeSO 4 ·7H 2 O...............................................................................0.5mg
ZnSO 4 ·7H 2 O..............................................................................0.3mg
H 3 BO 3 ........................................................................................0.1mg
CoCl 2 ·6H 2 O ...............................................................................0.1mg
CuSO 4 ·5H 2 O..............................................................................0.1mg
MnSO 4 ·4H 2 O.............................................................................0.1mg
Na 2 MoO 4 ·2H 2 O .........................................................................0.1mg
Preparation of Trace Elements: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Preparation of Medium: Add components, except glucose solution, to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.5. Autoclave for 15 min at 15 psi pressure121°C. Cool to 45°–50°C. Aseptically add sterile glucose solution. Mix thoroughly. Aseptically distribute into sterile tubes or flasks.
Use: For the cultivation of Saprospira thermalis, Flexibacter elegans,and Flexibacter rubrum.