FLN Medium

[所属分类:培养基配方] [发布时间:2021-3-1] [发布人: ] [阅读次数:] [返回]

FLN Medium
(Fluorescence Lactose Nitrate Medium)

培养基配方

Composition per liter:
Lactose........................................................................................20.0g
Agar............................................................................................15.0g
Proteose peptone No. 3...............................................................10.0g
KNO 3 ............................................................................................2.0g
K 2 HPO 4 .........................................................................................1.5g
MgSO 4 ·7H 2 O................................................................................1.5g
NaNO 2 ..........................................................................................0.5g
Phenol Red..................................................................................0.02g
pH 7.2 ±0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.
Use: For the differentiation of pseudomonads from other nonfermentative bacilli. Lactose fermentation is indicated by the medium turning yellow. Pseudomonas cepacia often produces acid from lactose. Denitrification from nitrate or nitrite is indicated by the formation of gas bubbles in the solid medium. Pseudomonas aeruginosa, Pseudomonas mendocina, and Pseudomonas denitrificans are positive for denitrification. Fluorescein production is indicated by fluorescence under UV light. Pseudomonas aeruginosa is positive for fluorescein production; Pseudomonas denitrificans does not produce fluorescein.