Fluconazole Testing Medium

[所属分类:培养基配方] [发布时间:2021-3-1] [发布人: ] [阅读次数:] [返回]

Fluconazole Testing Medium

培养基配方

Composition per liter:
Glucose .....................................................................................19.98g
(NH 4 ) 2 SO 4 ...................................................................................4.99g
KH 2 PO 4 .......................................................................................1.99g
MgSO 4 ·7H 2 O anhydrous.............................................................0.99g
L -Glutamine................................................................................0.58g
NaCl..............................................................................................0.2g
CaCl 2 ·2H 2 O...................................................................................0.2g
L -Lysine monohydrochloride....................................................0.073g
L -Isoleucine...............................................................................0.052g
L -Leucine..................................................................................0.052g
Threonine................................................................................0.0476g
Valine........................................................................................0.047g
L -Arginine monohydrochloride ................................................0.042g
L -Histidine.................................................................................0.023g
Tryptophan..................................................................................0.02g
DL -Methionine ........................................................................0.0189g
Inositol..................................................................................0.00397g
ZnSO 4 ·7H 2 O...........................................................................0.0014g
H 3 BO 3 ...................................................................................0.00099g
Nicotinic acid........................................................................0.00079g
Pyridoxine hydrochloride .....................................................0.00079g
Calcium  D -pantothenic acid..................................................0.00079g
Aneurine hydrochloride........................................................0.00079g
MnSO 4 ·2H 2 O........................................................................0.00079g
Na 2 MoO 4 ·2H 2 O ....................................................................0.00047g
p-Amino benzoic acid (PABA)...........................................0.000395g
Riboflavin ...........................................................................0.000395g
FeCl 3 ...................................................................................0.000395g
Folic acid.............................................................................0.000395g
KI............................................................................................0.0002g
CuSO 4 ·5H 2 O.........................................................................0.00012g
Biotin crystalline.................................................................0.000004g
Agar solution..........................................................................100.0mL
Source: This medium is available from HiMedia.
Agar Solution:
Composition per 100.0mL:
Agar ............................................................................................10.0g
Preparation of Agar Solution:  Add agar to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Adjust pH to 7.5 with phosphate buffer. Autoclave for 10 min at 10 psi pressure–115 °C. Cool to 50°C.
Preparation of Medium: Add components, except agar solution, to distilled/deionized water and bring volume to 900.0mL. Mix thoroughly. Filter sterilize. Aseptically add agar solution. Mix thoroughly. Pour into Petri dishes or aseptically distribute into sterile tubes.

Use: For fluconazole susceptibility testing using Candida species.