Fluid Selenite Cystine HiVeg Medium

[所属分类:培养基配方] [发布时间:2021-3-1] [发布人: ] [阅读次数:] [返回]

Fluid Selenite Cystine HiVeg Medium
(Selenite Cystine HiVeg Broth)

培养基配方

Composition per liter:
Na 2 HPO 4 .....................................................................................10.0g
Plant hydrolysate ..........................................................................5.0g
Lactose..........................................................................................4.0g
L -Cystine.....................................................................................0.01g
HNaO 3 Se......................................................................................4.0g
pH 7.0 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from HiMedia.
Caution:  Sodium selenite is toxic and a potential teratogen and care must be taken to avoid inhalation of the powdered dye, contact with the skin, or ingestion, especially in pregnant laboratory workers.
Preparation of Medium: Add sodium hydrogen selenite to distilled/deionized water and bring volume to 1.0L. Add remaining components. Mix thoroughly. Gently heat. Do not autoclave. Distribute into sterile tubes in 10.0mL volumes. Sterilize for 15 min at 0 psi pressure–100°C. Do not autoclave.
Use: For the isolation and cultivation of Salmonella species from feces, dairy products, and other specimens.