Fluid Tetrathionate HiVeg Medium

[所属分类:培养基配方] [发布时间:2021-3-1] [发布人: ] [阅读次数:] [返回]

Fluid Tetrathionate HiVeg Medium without Iodine and BG
(Tetrathionate HiVeg Broth Base without Iodine & BG)

培养基配方

Composition per liter:
Na 2 S 2 O 3 ......................................................................................30.0g
CaCO 3 .........................................................................................10.0g
Plant hydrolysate ..........................................................................2.5g
Plant peptone ................................................................................2.5g
Synthetic detergent .......................................................................1.0g

Iodine-potassium iodide solution.............................................20.0mL
Brilliant Green solution ...........................................................10.0mL
pH 8.4 ± 0.2 at 25°C
Source: This medium, without iodine-potassium iodine solution and Brilliant Green solution, is available as a premixed powder from HiMedia.
Preparation of Tetrathionate Broth Base: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. A slight precipitate will remain. Do not autoclave. Cool to 25°C. Store at 4°C.
Iodine-Potassium Iodide Solution:
Composition per 20.0mL:
Iodine, resublimed ........................................................................6.0g
KI..................................................................................................5.0g
Preparation of Iodine-Potassium Iodide Solution: Add KI to 5.0mL of sterile distilled/deionized water. Mix thoroughly. Add iodine. Mix thoroughly. Bring volume to 20.0mL with sterile distilled/deionized water.
Brilliant Green Solution:
Composition per 100.0mL:
Brilliant Green ..............................................................................0.1g
Preparation of Brilliant Green Solution:  Add Brilliant Green to sterile distilled/deionized water. Mix thoroughly.
Preparation of Medium: Add components, except iodine-potassium iodine soulution and Brilliant Green solution to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. A slight precipitate will remain. Do not autoclave. Cool to 25°C. Add 20.0mL of iodine-potassium iodide solution, and 10.0mL of Brilliant Green solution. Mix thoroughly. Aseptically distribute into tubes in 10.0mL volumes. Do not heat medium after it has been mixed.
Use: For the selective isolation and cultivation of Salmonella species from foods.