Fluorescent Pectolytic Agar

[所属分类:培养基配方] [发布时间:2021-3-2] [发布人: ] [阅读次数:] [返回]

Fluorescent Pectolytic Agar (FPA Medium)

培养基配方

Composition per liter:
Proteose peptone No. 3...............................................................20.0g
Agar ............................................................................................15.0g
Pectin ............................................................................................5.0g

K 2 HPO 4 .........................................................................................1.5g
MgSO 4 ·7H 2 O..............................................................................0.73g
Antibiotic solution...................................................................10.0mL
Antibiotic Solution:
Composition per 10.0mL:
Cycloheximide..........................................................................0.075g
Novobiocin ...............................................................................0.045g
Penicillin G............................................................................ 75,000U
Ethanol.......................................................................................1.0mL
Preparation of Antibiotic Solution: Add components to 1.0mL of ethanol. Mix thoroughly. Let stand for 30 min. Bring volume to 10.0mL with distilled/deionized water. Mix thoroughly. Filter sterilize.
Caution:  Cycloheximide is toxic. Avoid skin contact or aerosol formation and inhalation.
Preparation of Medium: Add components, except antibiotic solution, to distilled/deionized water and bring volume to 990.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add sterile antibiotic solution. Mix thoroughly. Pour into sterile Petri dishes.
Use: For the cultivation of fluorescent Pseudomonas species that are pectinolytic.