Folic Acid Assay Medium

[所属分类:培养基配方] [发布时间:2021-3-2] [发布人: ] [阅读次数:] [返回]

Folic Acid Assay Medium

培养基配方

Composition per liter:
Glucose.......................................................................................20.3g
Lactose........................................................................................20.0g
Sodium acetate............................................................................10.0g
Vitamin assay casamino acids ......................................................5.0g
K 2 HPO 4 .........................................................................................2.5g
Polysorbate 80 (Tween™ 80).......................................................2.0g
L- Alanine.......................................................................................0.4g
L- Asparagine.................................................................................0.2g
MgSO 4 ·7H 2 O................................................................................0.2g
Mercaptoacetic acid..................................................................0.125g
L- Tryptophan.................................................................................0.1g
MnSO 4 ·H 2 O................................................................................0.08g
Adenine.......................................................................................0.04g
Guanine.......................................................................................0.04g
Uracil..........................................................................................0.04g
(NH 4 ) 2 SO 4 ·FeSO 4 ·6H 2 O.............................................................0.03g
Glutathione ................................................................................5.0mg
Pyridoxine·HCl..........................................................................4.0mg
p-Aminobenzoic acid.................................................................2.0mg

Riboflavin ..................................................................................1.0mg
Calcium pantothenate ................................................................0.8mg
Niacin.........................................................................................0.8mg
Thiamine·HCl ............................................................................0.4mg
Biotin .......................................................................................0.02mg
Source : This medium is available as a premixed powder from BD Diagnostic Systems.
Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 0 psi pressure–100°C.
Use: For the determination of the folic acid content of pharmaceutical products and other materials based upon the extent of growth of the folic acid-requiring Lactobacillus test species.