Frankia Isolation Medium

[所属分类:培养基配方] [发布时间:2021-3-3] [发布人: ] [阅读次数:] [返回]

Frankia Isolation Medium 

培养基配方 

Composition per liter:
Sucrose........................................................................................40.0g
Ca(NO 3 ) 2 ·4H 2 O.........................................................................0.242g
KNO 3 ........................................................................................0.085g
KCl............................................................................................0.061g
MgSO 4 ·7H 2 O............................................................................0.042g
KH 2 PO 4 .......................................................................................0.02g
MnSO 4 ·H 2 O...............................................................................4.5mg
FeCl 3 ·6H 2 O................................................................................2.5mg
H 3 BO 3 ........................................................................................1.5mg
ZnSO 4 ·7H 2 O..............................................................................1.5mg
Nicotinic acid.............................................................................0.5mg
Pyridoxine·HCl..........................................................................0.5mg
Na 2 MoO 4 ·2H 2 O .......................................................................0.25mg
Thiamine·HCl ............................................................................0.1mg
CuSO 4 ·5H 2 O............................................................................0.04mg
Mannitol solution.....................................................................10.0mL
Supplement solution ................................................................10.0mL
pH 5.5 ± 0.2 at 25°C
Mannitol Solution:
Composition per 100.0mL:
Mannitol.................................................................................... 11.84g
Preparation of Mannitol Solution: Add mannitol to distilled/de-ionized water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize.
Supplement Solution:
Composition per 10.0mL:
L- Glutamic acid.........................................................................0.185g
L- Arginine.................................................................................0.174g
L- Glutamine ..............................................................................0.146g
L- Aspartic acid..........................................................................0.133g
L- Asparagine.............................................................................0.132g
Glycine......................................................................................0.075g
Urea.............................................................................................0.06g
Naphthaloneacetic acid..............................................................2.0mg
Zeatin..........................................................................................1.0μg
Preparation of Supplement Solution: Add components to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components, except mannitol solution and supplement solution, to distilled/deionized water and bring volume to 980.0mL. Mix thoroughly. Adjust pH to 5.5. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add 10.0mL of sterile mannitol solution and 10.0mL of sterile supplement solution. Mix thoroughly. Aseptically distribute into sterile tubes or flasks.
Use: For the isolation and cultivation of Frankia species from root nodules.