Fungi Kimmig Selective Agar

[所属分类:培养基配方] [发布时间:2021-3-5] [发布人: ] [阅读次数:] [返回]

Fungi Kimmig Selective Agar

培养基配方

Composition per liter:
Glucose.......................................................................................19.0g
Agar............................................................................................15.0g
Peptone .......................................................................................15.0g
NaCl..............................................................................................1.0g
Glycerol .....................................................................................5.0mL
Selective solution.....................................................................10.0mL
pH 6.5 ± 0.2 at 25°C
Selective Solution:
Composition per 10.0mL:
Novobiocin ...................................................................................0.1g
Colistin........................................................................................0.08g
Preparation of Selective Solution: Add components to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components, except selective solution, to distilled/deionized water and bring volume to 990.0mL. Mix thoroughly. Gently heat and bring to boiling. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add 10.0mL selective solution. Mix thoroughly. Distribute into tubes or flasks.
Use: For the cultivation, isolation, identification, and strain preservation of pathogenic fungi.