Fusibacter paucivorans Medium (DSMZ Medium 853)

[所属分类:培养基配方] [发布时间:2021-3-5] [发布人: ] [阅读次数:] [返回]

Fusibacter paucivorans Medium

(DSMZ Medium 853)

培养基配方

Composition per 1068.0mL:
NaCl............................................................................................30.0g
Na-thiosulfate·5H 2 O...................................................................3.16g
MgCl 2 ·6H 2 O .................................................................................3.0g
Yeast extract..................................................................................1.0g
Trypticase™..................................................................................1.0g
NH 4 Cl ...........................................................................................1.0g
KCl................................................................................................1.0g
Na-acetate·3H 2 O...........................................................................0.5g
Cysteine-HCl·H 2 O........................................................................0.5g
K 2 HPO 4 .........................................................................................0.3g
KH 2 PO 4 .........................................................................................0.3g
CaCl 2 ·2H 2 O ..................................................................................0.1g
Resazurin...................................................................................0.5mg
NaHCO 3 solution.....................................................................40.0mL
Glucose solution......................................................................18.0mL
Na 2 S·9H 2 O solution.................................................................10.0mL
Trace elements solution...........................................................10.0mL
pH 7.3 ± 0.2 at 25°C
Trace Elements Solution:
Composition per liter:
MgSO 4 ·7H 2 O................................................................................3.0g
Nitrilotriacetic acid.......................................................................1.5g
NaCl..............................................................................................1.0g
MnSO 4 ·2H 2 O................................................................................0.5g
CoSO 4 ·7H 2 O...............................................................................0.18g
ZnSO 4 ·7H 2 O...............................................................................0.18g
CaCl 2 ·2H 2 O...................................................................................0.1g
FeSO 4 ·7H 2 O..................................................................................0.1g
NiCl 2 ·6H 2 O...............................................................................0.025g
KAl(SO 4 ) 2 ·12H 2 O.......................................................................0.02g
H 3 BO 3 .........................................................................................0.01g
Na 2 MoO 4 ·4H 2 O ..........................................................................0.01g
CuSO 4 ·5H 2 O...............................................................................0.01g
Na 2 SeO 3 ·5H 2 O...........................................................................0.3mg
Preparation of Trace Elements Solution:  Add nitrilotriacetic acid to 500.0mL of distilled/deionized water. Dissolve by adjusting pH to 7.0 with KOH. Add remaining components. Add distilled/deionized water to 1.0L. Mix thoroughly.
NaHCO 3 Solution:
Composition per 100.0mL:
NaHCO 3 ......................................................................................10.0g
Preparation of NaHCO 3 Solution:  Add NaHCO 3 to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 25°C. Must be prepared freshly.
Na 2 S·9H 2 O Solution :
Composition per 10.0mL:
Na 2 S·9H 2 O....................................................................................0.3g
Preparation of Na 2 S·9H 2 O Solution : Add Na 2 S·9H 2 O to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Autoclave under 100% N 2 for 15 min at 15 psi pressure–121°C. Cool to room temperature.
Glucose Solution :
Composition per 50.0mL:
Glucose .......................................................................................10.0g
Preparation of Glucose Solution : Add glucose to distilled/deionized water and bring volume to 50.0mL. Mix thoroughly. Filter sterilize.
Preparation of Medium: Prepare and dispense medium under 80% N 2 + 20% CO 2  gas mixture. Add components, except glucose solution, NaHCO 3 solution, and Na 2 S·9H 2 O solution, to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.0. Sparge with 80% N 2 + 20% CO 2 . Autoclave for 15 min at 15 psi pressure–121°C. Cool to 25°C. Aseptically and anaerobically add 18.0mL sterile glucose solution, 10.0mL sterile Na 2 S·9H 2 O solution, and 40.0mL sterile NaHCO 3 solution. Mix thoroughly. Adjust pH to 7.3. Aseptically and anaerobically distribute into tubes or bottles.
Use:  For the cultivation of Fusibacter paucivorans.