Fusobacterium necrophorum Medium

[所属分类:培养基配方] [发布时间:2021-3-5] [发布人: ] [阅读次数:] [返回]

Fusobacterium necrophorum Medium

培养基配方

Composition per 500.0mL:
Pancreatic digest of casein..........................................................16.0g
Agar..............................................................................................8.3g
Biosate ..........................................................................................4.0g
MgSO 4 ..........................................................................................2.5g
Na 2 HPO 4 .......................................................................................2.5g
Thiotone........................................................................................2.0g
Glucose.........................................................................................0.5g
Egg yolk emulsion, 50%..........................................................45.0mL
Crystal Violet solution.............................................................25.0mL
Phenylethyl alcohol solution....................................................1.35mL
pH 7.3 ± 0.2 at 25°C
Egg Yolk Emulsion, 50%:
Composition per 100.0mL:
Chicken egg yolks............................................................................ 11
Whole chicken egg ............................................................................1
NaCl (0.9% solution)...............................................................50.0mL
Preparation of Egg Yolk Emulsion, 50%: Soak eggs with 1:100 dilution of saturated mercuric chloride solution for 1 min. Crack 11 eggs, separating yolks from whites. Mix egg yolks with 1 chicken egg. Beat to form emulsion. Combine 50.0mL of egg yolk emulsion and 50.0mL of 0.9% NaCl solution. Mix thoroughly.
Crystal Violet Solution:
Composition per 25.0mL:
Crystal Violet.......................................................................... 0.0115g
Preparation of Crystal Violet Solution: Aseptically add Crystal Violet to sterile distilled/deionized water and bring volume to 25.0mL. Mix thoroughly.
Phenylethyl Alcohol Solution:
Composition per 100.0mL:
Phenylethyl alcohol ....................................................................0.27g
Preparation of Phenylethyl Alcohol Solution: Aseptically add phenylethyl alcohol to sterile distilled/deionized water and bring volume to 100.0mL. Mix thoroughly.
Preparation of Medium: Add components—except egg yolk  emulsion, 50%, Crystal Violet solution, and phenylethyl alcohol solution—to distilled/deionized water and bring volume to 428.65mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add 45.0mL of sterile egg yolk emulsion, 50%, 25.0mL of Crystal Violet solution, and 1.35mL of sterile phenylethyl alcohol solution. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes.
Use: For the isolation and cultivation of Fusobacterium necrophorum.