Gardnerella vaginalis Selective Medium

[所属分类:培养基配方] [发布时间:2021-3-8] [发布人: ] [阅读次数:] [返回]

培养基配方

Composition per liter:
Columbia blood agar base .....................................................940.0mL
Rabbit or horse serum..............................................................50.0mL
Antibiotic inhibitor solution ....................................................10.0mL
pH 7.2 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from Oxoid Unipath.
Columbia Blood Agar Base:
Composition per liter:
Special peptone...........................................................................23.0g
Agar............................................................................................10.0g
NaCl..............................................................................................5.0g
Starch............................................................................................1.0g
Source: Columbia blood agar base is available as a premixed powder from Oxoid Unipath.
Preparation of Columbia Blood Agar Base: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat until boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C.
Antibiotic Inhibitor Solution:
Composition per 10.0mL:
Nalidixic acid............................................................................0.035g
Gentamicin sulfate.....................................................................4.0mg
Amphotericin B .........................................................................2.0mg
Ethanol.......................................................................................4.0mL
Preparation of Antibiotic Inhibitor Solution: Add components to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.
Preparation of Medium: To 940.0mL of cooled, sterile Columbia blood agar base, aseptically add 50.0mL of rabbit or horse blood serum and 10.0mL of sterile antibiotic inhibitor solution. Pour into sterile Petri dishes or distribute into sterile tubes.
Use: For the selective isolation, cultivation, and differentiation of Gardnerella vaginalis from clinical specimens, such as the vaginal discharge of patients with vaginitis. Gardnerella vaginalis exhibits β-hemolysis on this medium.