Gassner Lactose Agar

[所属分类:培养基配方] [发布时间:2021-3-8] [发布人: ] [阅读次数:] [返回]

培养基配方

Composition per liter:

Lactose........................................................................................50.0g
Agar ............................................................................................13.0g
Meat peptone.................................................................................7.0g
NaCl..............................................................................................5.0g
Metachrome Yellow....................................................................1.25g
Water Blue ................................................................................0.625g
pH 7.2 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from HiMedia.
Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes.
Use: For the detection and isolation of pathogenic Enterobacteriaceae.