GC Agar with Supplement B

[所属分类:培养基配方] [发布时间:2021-3-9] [发布人: ] [阅读次数:] [返回]

GC Agar with Supplement B

培养基配方

GC agar base..........................................................................990.0mL
Supplement B...........................................................................10.0mL
pH 7.2 ± 0.2 at 25°C
GC Agar Base:
Composition per liter:
Agar ............................................................................................10.0g
Pancreatic digest of casein............................................................7.5g
Peptic digest of animal tissue........................................................7.5g
NaCl..............................................................................................5.0g
K 2 HPO 4 .........................................................................................4.0g
Cornstarch.....................................................................................1.0g
KH 2 PO 4 .........................................................................................1.0g
Source:  GC agar base is available as a premixed powder from BD Diagnostic Systems. This base may be replaced by GC medium base available from BD Diagnostic Systems.
Preparation of GC Agar Base: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat until boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C.
Supplement B:
Composition per 10.0mL:
Supplement B contains yeast concentrate, glutamine, coenzyme, cocarboxylase, hematin, and growth factors.
Preparation of Supplement B:  Add components to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.
Preparation of Medium: To 990.0mL of sterile GC agar base, aseptically add 10.0mL of sterile defined supplement B. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes.
Use:  For the cultivation and maintenance of Neisseria gonorrhoeae.