GCA Agar with Thiamine

[所属分类:培养基配方] [发布时间:2021-3-9] [发布人: ] [阅读次数:] [返回]

GCA Agar with Thiamine

培养基配方

Composition per liter:
Glucose .......................................................................................25.0g
Agar ............................................................................................14.0g
Papaic digest of soybean meal....................................................10.0g
NaCl..............................................................................................5.0g
Pancreatic digest of heart muscle..................................................3.0g
Cysteine·HCl·H 2 O.........................................................................1.0g
Thiamine..................................................................................0.05mg
Rabbit blood, defibrinated .......................................................50.0mL
pH 6.8 ± 0.2 at 25°C
Preparation of Medium: Add components, except rabbit blood, to distilled/deionized water and bring volume to 950.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add sterile rabbit blood. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes.
Use: For the isolation and cultivation of Francisella tularensis.