Gelatin Agar (GA Medium)

[所属分类:培养基配方] [发布时间:2021-3-9] [发布人: ] [阅读次数:] [返回]

Gelatin Agar

(GA Medium)

培养基配方

Composition per liter:
Solution 1...............................................................................950.0mL
Solution 2.................................................................................50.0mL
pH 7.2 ± 0.2 at 25°C
Solution 1:
Composition per 950.0mL:
Gelatin.........................................................................................30.0g
Agar............................................................................................15.0g
Pancreatic digest of casein..........................................................10.0g
NaCl..............................................................................................2.0g
D- Mannitol ....................................................................................1.0g
Glucose.........................................................................................1.0g
KNO 3 ............................................................................................1.0g
Sodium acetate..............................................................................1.0g
Sodium formate ............................................................................1.0g
Sodium succinate..........................................................................1.0g
Yeast extract..................................................................................1.0g
Sodium lactate (60% solution)...................................................5.0mL
Preparation of Solution 1:  Add components to distilled/deionized water and bring volume to 950.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C.
Solution 2:
Composition per 50.0mL:
Na 2 HPO 4 .......................................................................................1.0g
L- Cysteine·HCl·H 2 O .....................................................................0.5g
Na 2 CO 3 ·H 2 O.................................................................................0.5g
Sucrose..........................................................................................0.5g
Dithiothreitol.................................................................................0.1g
Menadione solution....................................................................2.0mL
Preparation of Solution 2:  Add components to distilled/deionized water and bring volume to 50.0mL. Mix thoroughly. Filter sterilize.
Menadione Solution:
Composition per 100.0mL:
Menadione (vitamin K 3 ).............................................................0.05g
Ethanol.....................................................................................99.0mL
Preparation of Menadione Solution:  Add menadione to 99.0mL of absolute ethanol. Mix thoroughly.
Preparation of Medium: Aseptically combine sterile solution 1 with sterile solution 2. Mix thoroughly. Pour into sterile Petri dishes.
Use: For the cultivation and differentiation of microorganisms from dental plaque based on their ability to produce gelatinase. For the differentiation of aerobic, anaerobic, and facultative microorganisms of clinical significance.