Gelatin Infusion Broth

[所属分类:培养基配方] [发布时间:2021-3-10] [发布人: ] [阅读次数:] [返回]

Gelatin Infusion Broth

培养基配方

Composition per liter:
Beef heart, solids from infusion................................................500.0g
Gelatin.........................................................................................40.0g
Tryptose ......................................................................................10.0g
NaCl..............................................................................................5.0g
pH 7.4 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.
Use: For the cultivation and differentiation of a variety of heterotrophic bacteria based upon their production of gelatinase. The gelatinase liquefies the medium.