Gelatinase Test Medium

[所属分类:培养基配方] [发布时间:2021-3-10] [发布人: ] [阅读次数:] [返回]

Gelatinase Test Medium

培养基配方

Composition per liter:
Gelatin...........................................................................................3.0g
ACES buffer .................................................................................1.0g
Yeast extract..................................................................................1.0g
Charcoal, activated .....................................................................0.15g
α-Ketoglutarate monopotassium salt............................................0.1g
L- Cysteine·HCl·H 2 O (4% solution)............................................1.0mL
KOH (85% solution)..................................................................1.0mL
Fe 4 (P 2 O 7 ) 3 solution....................................................................1.0mL
pH 6.9 ± 0.2 at 25°C
L- Cysteine·HCl·H 2 O Solution:
Composition per 10.0mL:
L- Cysteine·HCl·H 2 O .....................................................................0.4g
Preparation of  L- Cysteine·HCl·H 2 O Solution: Add  Lcysteine·HCl·H 2 O to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.
Fe 4 (P 2 O 7 ) 3 Solution:
Composition per 10.0mL:
Fe 4 (P 2 O 7 ) 3 ...................................................................................0.15g

Preparation of Fe 4 (P 2 O 7 ) 3 Solution: Add Fe 4 (P 2 O 7 ) 3 to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add ACES buffer to distilled/deionized water and bring volume to 899.0mL. Mix thoroughly. Gently heat to 50°C. Add 1.0mL of KOH solution. Mix thoroughly. Add charcoal, yeast extract, and α-ketoglutarate. Add 80.0mL of distilled/deionized water to wash sides of flask. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°C. Aseptically add 10.0mL of sterile cysteine solution and 10.0mL of sterile Fe 4 (P 2 O 7 ) 3 solution. Mix thoroughly. Adjust pH to 6.9. Aseptically distribute into sterile screwcapped tubes.
Use: For the cultivation and differentiation of gelatinase-producing bacteria.