Geobacter Medium (DSMZ Medium 579)

[所属分类:培养基配方] [发布时间:2021-3-10] [发布人: ] [阅读次数:] [返回]

Geobacter Medium

(DSMZ Medium 579)

培养基配方

Composition per liter:
Fe(III) citrate...............................................................................13.7g
NaHCO 3 ........................................................................................2.5g
Na-acetate.....................................................................................2.5g
NH 4 Cl ...........................................................................................1.5g
NaH 2 PO 4 .......................................................................................0.6g
KCl................................................................................................0.1g
Na 2 WO 4 ·2H 2 O.........................................................................0.25mg
Vitamin solution.......................................................................10.0mL
Trace elements solution...........................................................10.0mL
pH 6.7–7.0 at 25°C
Trace Elements Solution:
Composition per liter:
MgSO 4 ·7H 2 O................................................................................3.0g
Nitrilotriacetic acid.......................................................................1.5g
NaCl..............................................................................................1.0g
MnSO 4 ·2H 2 O................................................................................0.5g
CoSO 4 ·7H 2 O...............................................................................0.18g
ZnSO 4 ·7H 2 O...............................................................................0.18g
CaCl 2 ·2H 2 O ..................................................................................0.1g
FeSO 4 ·7H 2 O..................................................................................0.1g
NiCl 2 ·6H 2 O...............................................................................0.025g
KAl(SO 4 ) 2 ·12H 2 O.......................................................................0.02g
H 3 BO 3 .........................................................................................0.01g
Na 2 MoO 4 ·4H 2 O..........................................................................0.01g
CuSO 4 ·5H 2 O...............................................................................0.01g
Na 2 SeO 3 ·5H 2 O...........................................................................0.3mg
Preparation of Trace Elements Solution:  Add nitrilotriacetic acid to 500.0mL of distilled/deionized water. Dissolve by adjusting pH to 6.5 with KOH. Add remaining components. Add distilled/deionized water to 1.0L. Mix thoroughly.
Vitamin Solution:
Composition per liter:
Pyridoxine-HCl........................................................................10.0mg
Thiamine-HCl·2H 2 O..................................................................5.0mg
Riboflavin..................................................................................5.0mg
Nicotinic acid.............................................................................5.0mg
D -Ca-pantothenate .....................................................................5.0mg
p-Aminobenzoic acid.................................................................5.0mg
Lipoic acid.................................................................................5.0mg
Biotin.........................................................................................2.0mg
Folic acid ...................................................................................2.0mg
Vitamin B 12 ................................................................................0.1mg
Preparation of Vitamin Solution:  Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Sparge with 80% H 2 + 20% CO 2 . Filter sterilize.
Preparation of Medium: Prepare and dispense medium under an oxygen-free 80% N 2 + 20% CO 2  gas mixture. Dissolve ferric citrate in 900.0mL distilled/deionized water by heating and adjust to pH 6.0. Add other components, except vitamin solution, to distilled/deionized water and bring volume to 990.0mL. Mix thoroughly. Sparge with 80% N 2 + 20% CO 2 . Autoclave for 15 min at 15 psi pressure–121°C. Cool to 25°C. Aseptically and anaerobically add 10.0mL vitamin solution. Mix thoroughly. Aseptically and anaerobically distribute into sterile tubes or flasks.

Use:  For the cultivation of Geobacter metallireducens and Geobacter grbiciae.