Geobacter Medium

[所属分类:培养基配方] [发布时间:2021-3-10] [发布人: ] [阅读次数:] [返回]

Geobacter Medium

培养基配方

Composition per liter:
Ferric citrate................................................................................13.7g
Sodium acetate..............................................................................2.5g
NaHCO 3 ........................................................................................2.5g
NH 4 Cl ...........................................................................................1.5g
NaH 2 PO 4 .......................................................................................0.6g
KCl................................................................................................0.1g
Na 2 WO 4 ·2H 2 O.........................................................................0.25mg
Trace elements solution...........................................................10.0mL
Vitamin solution.......................................................................10.0mL
pH 6.7–7.0 at 25°C
Trace Elements Solution:
Composition per liter:
MgSO 4 ·7 H 2 O...............................................................................3.0g
Nitrilotriacetic acid.......................................................................1.5g
CaCl 2 ·2 H 2 O.................................................................................1.0g
NaCl..............................................................................................1.0g
MnSO 4 ·2 H 2 O...............................................................................0.5g
CoSO 4 ·7 H 2 O..............................................................................0.18g
ZnSO 4 ·7 H 2 O..............................................................................0.18g
FeSO 4 ·7 H 2 O................................................................................0.1g
NiCl 2 ·6 H 2 O..............................................................................0.025g
KAI(SO 4 ) 2 ·12 H 2 O.....................................................................0.02g
CuSO 4 ·5 H 2 O..............................................................................0.01g
H 3 BO 3 .........................................................................................0.01g
Na 2 MoO 4 ·2 H 2 O.........................................................................0.01g

Na 2 SeO 3 ·5 H 2 O.........................................................................0.3 mg

Preparation of Trace Elements Solution : Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH. Add minerals. Adjust final pH to 7.0 with KOH.
Vitamin Solution:
Composition per liter:
Pyridoxine-HCl........................................................................10.0mg
Calcium  DL -pantothenate...........................................................5.0mg
Lipoic acid .................................................................................5.0mg
Nicotinic acid.............................................................................5.0mg
p-Aminobenzoic acid.................................................................5.0mg
Riboflavin ..................................................................................5.0mg
Thiamine-HCl............................................................................5.0mg
Biotin .........................................................................................2.0mg
Folic acid....................................................................................2.0mg
Vitamin B 12 ................................................................................0.1mg
Preparation of Vitamin Solution : Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Preparation of Medium: Prepare and dispense medium under 80% N 2 + 20% CO 2 . Add ferric citrate to distilled/deionized water and bring volume to 500.0mL. Mix thoroughly. Gently heat until ferric citrate dissolves. Adjust pH to 6.0. Add remaining components. Add distilled/ deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.
Use: For the cultivation and maintenance of Geobacter metallireducens.