Geococcus nitratireducens and Petrobacter succinimandens Medium (DSMZ Medium 979)

[所属分类:培养基配方] [发布时间:2021-3-10] [发布人: ] [阅读次数:] [返回]

Geococcus nitratireducens and
Petrobacter succinimandens Medium

(DSMZ Medium 979)

培养基配方

Composition per liter:
Glucose.........................................................................................3.6g
NaCl..............................................................................................1.0g
NH 4 Cl ..........................................................................................1.0g
Yeast extract..................................................................................1.0g
KH 2 PO 4 .........................................................................................0.3g
K 2 HPO 4 .........................................................................................0.3g
MgCl 2 · 6H 2 O .................................................................................0.2g
Sodium acetate............................................................................0.16g
CaCl 2 ·2H 2 O ..................................................................................0.1g
KCl................................................................................................0.1g
Trace elements solution SL-10................................................10.0mL
Vitamin solution.........................................................................5.0mL
pH 6.8 ± 0.2 at 25°C
Trace Elements Solution SL-10:
Composition per liter:
MgSO 4 ·7H 2 O................................................................................3.0g
Nitrilotriacetic acid.......................................................................1.5g
NaCl..............................................................................................1.0g
MnSO 4 ·2H 2 O................................................................................0.5g
CoSO 4 ·7H 2 O...............................................................................0.18g
ZnSO 4 ·7H 2 O...............................................................................0.18g
CaCl 2 ·2H 2 O ..................................................................................0.1g

FeSO 4 ·7H 2 O..................................................................................0.1g

NiCl 2 ·6H 2 O...............................................................................0.025g
KAl(SO 4 ) 2 ·12H 2 O.......................................................................0.02g
H 3 BO 3 .........................................................................................0.01g
Na 2 MoO 4 ·4H 2 O ..........................................................................0.01g
CuSO 4 ·5H 2 O...............................................................................0.01g
Na 2 SeO 3 ·5H 2 O...........................................................................0.3mg
Preparation of Trace Elements Solution SL-10:  Add nitrilotriacetic acid to 500.0mL of distilled/deionized water. Dissolve by adjusting pH to 6.5 with KOH. Add remaining components. Add distilled/deionized water to 1.0L. Mix thoroughly.
Vitamin Solution:
Composition per liter:
Pyridoxine-HCl........................................................................10.0mg
Thiamine-HCl·2H 2 O..................................................................5.0mg
Riboflavin ..................................................................................5.0mg
Nicotinic acid.............................................................................5.0mg
D -Ca-pantothenate......................................................................5.0mg
p-Aminobenzoic acid.................................................................5.0mg
Lipoic acid .................................................................................5.0mg
Biotin .........................................................................................2.0mg
Folic acid....................................................................................2.0mg
Vitamin B 12 ................................................................................0.1mg
Preparation of Vitamin Solution:  Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Sparge with 80% H 2 + 20% CO 2 . Filter sterilize.
Preparation of Medium:  Add components, except vitamin solution, to distilled/deionized water and bring volume to 1.0L. Gently heat and bring to boiling. Mix thoroughly. Cool to room temperature while sparging with 80% N 2  + 20% CO 2 . Dispense into culture vessels under an atmosphere of 80% N 2  + 20% CO 2 . Autoclave for 45 min at 6 psi pressure–110°C. Aseptically and anoxically add vitamin solution.
Use: For the cultivation of Geococcus nitratireducens.