Gillies Agar No. 2 (Sucrose Salicin Agar)

[所属分类:培养基配方] [发布时间:2021-3-11] [发布人: ] [阅读次数:] [返回]

Gillies Agar No. 2

(Sucrose Salicin Agar)

培养基配方

Composition per liter:
Peptic digest of animal tissue......................................................10.0g
Casein enzymic hydrolysate .......................................................10.0g
NaCl..............................................................................................5.0g
Na 2 H 2 PO 4 ....................................................................................0.25g
Sucrose........................................................................................10.0g
Salicin .........................................................................................10.0g
Bromthymol Blue .......................................................................0.01g
Na 2 S 2 O 3 ....................................................................................0.025g
Agar ..............................................................................................3.0g
pH 7.4 ± 0.2 at 25°C
Source: This medium is available from HiMedia.
Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes. Autoclave for 15 min at 15 psi pressure–121°C. Allow the tubes to cool in an upright position. Suspend Kovacs reagent strips and lead acetate papers from the cap or the cotton plug over the medium but not touching the surface of the medium.
Use: For the detection of motility, hydrogen sulphide, indole production, and fermentation of sucrose and salicin for identification of Salmonella spp. and Shigella spp.