Gluconate Peptone Broth

[所属分类:培养基配方] [发布时间:2021-3-11] [发布人: ] [阅读次数:] [返回]

Gluconate Peptone Broth

培养基配方

Composition per liter:
Potassium gluconate ...................................................................40.0g
Casein peptone..............................................................................1.5g
K 2 HPO 4 .........................................................................................1.0g
Yeast extract..................................................................................1.0g
pH 7.0 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.
Use: For the cultivation and differentiation of Gram-negative bacteria based on their ability to oxidize gluconate to 2-ketogluconate. For the differentiation of fluorescent Pseudomonas species. After inoculation with bacteria and 48 hr of growth in this medium, Benedict’s reagent is added. Bacteria that produce the reducing sugar 2-ketogluconate turn the reagent yellow-orange to orange-red.