Glucose Agar, 9K

[所属分类:培养基配方] [发布时间:2021-3-12] [发布人: ] [阅读次数:] [返回]

Glucose Agar, 9K

培养基配方

Composition per liter:
(NH 4 ) 2 SO 4 ....................................................................................3.0g
KH 2 PO 4 .........................................................................................0.5g
MgSO 4 ·7H 2 O................................................................................0.5g
KCl................................................................................................0.1g
Ca(NO 3 ) 2 .................................................................................0.0125g
FeSO 4 ·7H 2 O.............................................................................0.01mg
Agar solution .........................................................................500.0mL
Glucose solution....................................................................100.0mL
pH 4.5 ± 0.2 at 25°C
Agar Solution:
Composition per 500.0mL:
Agar............................................................................................15.0g
Preparation of Agar Solution: Add agar to distilled/deionized water and bring volume to 500.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 55°C.
Glucose Solution:
Composition per 100.0mL:
Glucose.......................................................................................10.0g
Preparation of Glucose Solution: Add glucose to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components, except agar solution and glucose solution, to distilled/deionized water and bring volume to 400.0mL. Mix thoroughly. Adjust pH to 4.5 with H 2 SO 4 . Autoclave for 15 min at 15 psi pressure–121°C. Cool to 55°C. Aseptically add 500.0mL of sterile agar solution and 100.0mL of sterile glucose solution. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes.

Use:  For the cultivation of Thiobacillus acidophilus.