Glucose Azide Broth

[所属分类:培养基配方] [发布时间:2021-3-12] [发布人: ] [阅读次数:] [返回]

Glucose Azide Broth

培养基配方

Composition per liter:
Peptic digest of animal tissue......................................................10.0g
Glucose .........................................................................................5.0g
K 2 HPO 4 .........................................................................................5.0g
NaCl..............................................................................................5.0g
Yeast extract..................................................................................3.0g
KH 2 PO 4 .........................................................................................2.0g
NaN 3 ...........................................................................................0.25g
Bromcresol Purple ......................................................................0.03g
pH 6.7 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from HiMedia.

Caution: Sodium azide is toxic. Azides also react with metals and disposal must be highly diluted.
Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.
Use: For the enumeration of fecal streptococci by the MPN technique from water and sewage.