Glucose Medium Nakayama (DSMZ Medium 452)

[所属分类:培养基配方] [发布时间:2021-3-12] [发布人: ] [阅读次数:] [返回]

Glucose Medium Nakayama

(DSMZ Medium 452)

培养基配方

Composition per liter:
Yeast extract................................................................................15.0g
Glucose .......................................................................................10.0g

Peptone........................................................................................10.0g

Agar............................................................................................10.0g
Solution A................................................................................10.0mL
Solution B................................................................................10.0mL
Solution C..................................................................................1.0mL
pH 6.8 ± 0.2 at 25°C
Solution A:
Composition per 100.0mL:
KH 2 PO 4 .........................................................................................0.5g
K 2 HPO 4 .........................................................................................0.5g
Preparation of Solution A: Add components to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°C.
Solution B:
Composition per 100.0mL:
MgSO 4 ·7H 2 O................................................................................3.0g
NaCl..............................................................................................0.1g
MnSO 4 ·5H 2 O................................................................................0.1g
CuSO 4 ·5H 2 O...............................................................................0.01g
Preparation of Solution B: Add components to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°C.
Solution C:
Composition per 100.0mL:
Na 3 -citrate.....................................................................................2.0g
FeSO 4 ·7H 2 O..................................................................................0.1g
Preparation of Solution C: Add components to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°C.
Preparation of Medium: Add components, except solutions A, B, and C, to distilled/deionized water and bring volume to 979.0mL. Mix thoroughly. Adjust pH to 6.8. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°C. Aseptically add 10.0mL sterile solution A, 10.0mL sterile solution B, and 1.0mL sterile solution C. Mix thoroughly. Pour into sterile Petri dishes or aseptically dispense into sterile tubes.
Use: For the growth and maintenance of Bacillus laevolacticus.