Glucose Salts Medium

[所属分类:培养基配方] [发布时间:2021-3-15] [发布人: ] [阅读次数:] [返回]

Glucose Salts Medium

培养基配方

Composition per 1000.5mL:
Glucose .........................................................................................5.0g
(NH 4 ) 2 SO 4 .....................................................................................1.0g
MgSO 4 ·7H 2 O................................................................................0.5g
NaCl..............................................................................................0.5g
NaH 2 PO 4 ·12H 2 O........................................................................0.7mg
NaH 2 PO 4 ·2H 2 O..........................................................................0.3mg
Trace elements solution .............................................................0.5mL
pH 6.9 ± 0.2 at 25°C
Trace Elements Solution:
Composition per liter:
H 3 BO 3 ...........................................................................................0.3g
CoCl 2 ·6H 2 O ..................................................................................0.2g
ZnSO 4 ·7H 2 O.................................................................................0.1g
MnCl 2 ·4H 2 O................................................................................0.03g
Na 2 MoO 4 ·2H 2 O ..........................................................................0.03g
NiCl 2 ·6H 2 O.................................................................................0.02g
CuCl 2 ·2H 2 O ................................................................................0.01g
Preparation of Trace Elements Solution:  Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Filter sterilize.
Preparation of Medium:  Add components, except trace elements solution, to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Aseptically add 0.5mL of sterile trace elements solution. Mix thoroughly. Aseptically distribute into sterile tubes or flasks.

Use: For the cultivation of a wide variety of bacteria and fungi.