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培养基配方
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Glucose Yeast Extract Peptone Agar with 2% Glucose
[所属分类:培养基配方] [发布时间:2021-3-15] [发布人: ] [阅读次数:] [返回]
Glucose Yeast Extract
Glucose .......................................................................................20.0g
Agar ............................................................................................15.0g
Peptone..........................................................................................5.0g
Yeast extract..................................................................................1.0g
K 2 HPO 4 .........................................................................................0.5g
MgSO 4 ·7H 2 O................................................................................0.5g
CaCO 3 ...........................................................................................1.0g
FeSO 4 ·7H 2 O.............................................................................50.0mg
pH 6.8 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 6.8. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.
Use: For the cultivation and maintenance of Lactobacillus acidophilus and Leclercia adecarboxylata.
Peptone Agar with 2% Glucose
培养基配方
Composition per liter:Glucose .......................................................................................20.0g
Agar ............................................................................................15.0g
Peptone..........................................................................................5.0g
Yeast extract..................................................................................1.0g
K 2 HPO 4 .........................................................................................0.5g
MgSO 4 ·7H 2 O................................................................................0.5g
CaCO 3 ...........................................................................................1.0g
FeSO 4 ·7H 2 O.............................................................................50.0mg
pH 6.8 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 6.8. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.
Use: For the cultivation and maintenance of Lactobacillus acidophilus and Leclercia adecarboxylata.