Glutamate Starch Phenol Red Agar Base

[所属分类:培养基配方] [发布时间:2021-3-17] [发布人: ] [阅读次数:] [返回]

Glutamate Starch Phenol Red Agar Base

培养基配方

Composition per liter:
Starch, soluble.............................................................................20.0g
L -Glutamate, sodium...................................................................10.0g
Agar............................................................................................12.0g
KH 2 PO 4 .........................................................................................2.0g
MgSO 4 ·7H 2 O................................................................................0.5g
Phenol Red..................................................................................0.36g
Selective supplement solution .................................................10.0mL
pH 7.2 ± 0.2 at 25°C
Source: This medium is available from HiMedia.
Selective Supplement Solution:
Composition per 10.0mL:
Penicillin G100,00 U Preparation of Selective Supplement Solution:  Add components to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components, except selective supplement solution, to distilled/deionized water and bring volume to 990.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°C. Aseptically add selective supplement solution. Mix thoroughly. Pour into Petri dishes or aseptically distribute into sterile tubes.
Use: For the detection of Pseudomonas spp. and Aeromonas spp. in foodstuffs, wastewater, and equipment, in the food industry.