Glycerol-Free Medium

[所属分类:培养基配方] [发布时间:2021-3-18] [发布人: ] [阅读次数:] [返回]

Glycerol-Free Medium

培养基配方

Composition per liter:
L- Asparagine.................................................................................4.0g
L- Glutamine ..................................................................................4.0g
Monosodium glutamate................................................................4.0g
Na 2 HPO 4 .......................................................................................2.5g
Pancreatic digest of casein............................................................1.0g
KH 2 PO 4 .........................................................................................1.0g
Triton ® WR 1339.........................................................................0.25g
Ferric ammonium citrate.............................................................0.05g
CaCl 2 ..........................................................................................1.0mg
CuSO 4 ........................................................................................0.5mg
ZnSO 4 ........................................................................................0.5mg
Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.
Use: For the cultivation of Mycobacterium tuberculosis Bacille Calmette-Guèrin (BCG) for vaccine production.