GN Broth, Hajna

[所属分类:培养基配方] [发布时间:2021-3-18] [发布人: ] [阅读次数:] [返回]

GN Broth, Hajna

培养基配方

Composition per liter:
Tryptose ......................................................................................20.0g
NaCl..............................................................................................5.0g
Sodium citrate...............................................................................5.0g
K 2 HPO 4 .........................................................................................4.0g
Mannitol........................................................................................2.0g
KH 2 PO 4 .........................................................................................1.5g
Glucose .........................................................................................1.0g
Sodium deoxycholate....................................................................0.5g
pH 7.0 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from HiMedia.
Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 10 psi pressure–115°C.
Use: For the selective cultivation of Salmonella and Shigella species.