GNYS Agar (DSMZ Medium 1119)

[所属分类:培养基配方] [发布时间:2021-3-18] [发布人: ] [阅读次数:] [返回]

GNYS Agar

(DSMZ Medium 1119)

培养基配方

Composition per liter:
Agar............................................................................................18.0g
Sodium gluconate ........................................................................3.0g
Yeast extract .................................................................................1.0g
(NH 4 ) 2 SO 4 ....................................................................................1.0g
KH 2 PO 4 .........................................................................................1.0g
NaCl..............................................................................................0.2g
MgCl 2 · 6H 2 O .................................................................................0.2g
CaCl 2 ·2H 2 O ................................................................................0.05g
Trace elements solution SL-8....................................................1.0mL
pH 5.0 ± 0.2 at 25°C
Trace Elements Solution SL-8:
Composition per liter:
Disodium EDTA...........................................................................5.2g
FeCl 2 ·4H 2 O...................................................................................1.5g
CoCl 2 ·6H 2 O................................................................................0.19g
MnCl 2 ·4H 2 O .................................................................................0.1g
ZnCl 2 ...........................................................................................0.07g
H 3 BO 3 .........................................................................................0.06g
NaMoO 4 ·2H 2 O............................................................................0.04g
CuCl 2 ·2H 2 O................................................................................0.02g
NiCl 2 ·6H 2 0..................................................................................0.02g
Preparation of Trace Elements Solution SL-8: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 5.0. Gently heat while stirring and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into Petri dishes or leave in tubes.
Use: For the cultivation of Acidisphaera rubrifaciens.