Gonococcus Medium

[所属分类:培养基配方] [发布时间:2021-3-18] [发布人: ] [阅读次数:] [返回]

Gonococcus Medium

培养基配方

Composition per 623.0mL:
Part II.....................................................................................500.0mL
Part I.......................................................................................123.0mL
pH 7.4 ± 0.2 at 25°C
Part I:
Composition per 123.0mL:
K 2 HPO 4 .......................................................................................10.5g
Glucose.........................................................................................7.5g
NaCl............................................................................................5.25g
KH 2 PO 4 .........................................................................................4.5g
Sodium acetate..............................................................................1.5g
L- Cysteine·HCl·H 2 O .....................................................................1.2g
Sodium citrate.............................................................................1.13g
NaHCO 3 ........................................................................................1.0g
K 2 SO 4 ...........................................................................................0.9g
Na 2 SO 4 ........................................................................................0.75g
MgCl 2 ·6H 2 O ...............................................................................0.45g
KCl................................................................................................0.3g
NH 4 Cl ...........................................................................................0.3g
L- Arginine·HCl............................................................................0.25g
L- Proline......................................................................................0.25g
Oxaloacetate ...............................................................................0.25g
L- Glutamic acid...........................................................................0.19g
L- Methionine...............................................................................0.19g
L- Asparagine·H 2 O.......................................................................0.13g
L- Isoleucine.................................................................................0.13g
L- Serine.......................................................................................0.13g
L- Cystine.....................................................................................0.05g
Calcium pantothenate .................................................................0.02g
Thiamine·HCl.............................................................................0.02g
Thiamine pyrophosphate chloride ..............................................0.02g
Nicotinamide adenine dinucleotide ............................................0.01g
CaCl 2 ·2H 2 O ...............................................................................5.0mg
Fe(NO 3 ) 3 ·9H 2 O..........................................................................5.0mg
Uracil.........................................................................................5.0mg
Biotin.........................................................................................4.0mg
Hypoxanthine.............................................................................2.5mg

Sodium thioglycolate.............................................................0.025mg

Preparation of Part I: Add components to distilled/deionized water and bring volume to 123.0mL. Mix thoroughly. Adjust pH to 7.2 with 6N NaOH. Warm to 50°C for 45 min. Filter sterilize.
Part II:
Composition per 500.0mL:
Agar ............................................................................................10.0g
Soluble starch................................................................................7.5g
Preparation of Part II: Add components to distilled/deionized water and bring volume to 500.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C.
Preparation of Medium: Aseptically combine 123.0mL of sterile part I and 500.0mL of cooled, sterile part II. Mix thoroughly. Pour into sterile Petri dishes.
Use: For the cultivation of Neisseria gonorrhoeae.