Grace's Insect Medium

[所属分类:培养基配方] [发布时间:2021-3-19] [发布人: ] [阅读次数:] [返回]

Grace's Insect Medium

培养基配方

Composition per liter:
Sucrose......................................................................................26.68g

KCl................................................................................................4.1g

MgCl 2 ·6H 2 O ...............................................................................2.28g
MgSO 4 ·7H 2 O..............................................................................2.78g
L -Histidine ....................................................................................2.5g
DL -Serine ......................................................................................1.1g
NaH 2 PO 4 ·H 2 O...........................................................................1.013g
CaCl 2 ...........................................................................................0.75g
L -Leucine....................................................................................0.75g
D -Glucose......................................................................................0.7g
L -Arginine·HCl.............................................................................0.7g
Malic acid...................................................................................0.67g
Glycine........................................................................................0.65g
L -Lysine·HCl.............................................................................0.625g
L -Glutamic acid ............................................................................0.6g
L -Glutamine..................................................................................0.6g
Fructose.........................................................................................0.4g
α-Ketoglutaric acid.....................................................................0.37g
NaHCO 3 ......................................................................................0.35g
L -Asparagine...............................................................................0.35g
L -Aspartic acid............................................................................0.35g
L -Proline .....................................................................................0.35g
L -Alanine ..................................................................................0.225g
β-Alanine......................................................................................0.2g
Choline chloride.........................................................................0.2mg
L -Threonine...............................................................................0.175g
L -Phenylalanine ..........................................................................0.15g
L -Tryptophan ................................................................................0.1g
L -Tyrosine.....................................................................................0.1g
L -Valine.........................................................................................0.1g
Succinic acid...............................................................................0.06g
Fumaric acid.............................................................................0.055g
L -Isoleucine.................................................................................0.05g
L -Methionine...............................................................................0.05g
L -Cysteine.................................................................................0.022g
p-Aminobenzoic acid...............................................................0.02mg
Calcium  DL -pantothenate.........................................................0.02mg
Folic acid .................................................................................0.02mg
i-Inositol...................................................................................0.02mg
Nicotinic acid...........................................................................0.02mg
Pyridoxine·HCl........................................................................0.02mg
Riboflavin................................................................................0.02mg
Thiamine·HCl..........................................................................0.02mg
Biotin.......................................................................................0.01mg
Source: Grace’s Insect Medium is available from BD Diagnostics. 

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Filter sterilize. Aseptically distribute into sterile tubes or flasks.
Use: For the cultivation of Blastocrithidia culicis.