GTC Agar Base with Bicarbonate

[所属分类:培养基配方] [发布时间:2021-3-19] [发布人: ] [阅读次数:] [返回]

GTC Agar Base with Bicarbonate

培养基配方

Composition per liter:
Agar ............................................................................................15.0g
Casein enzymatic hydrolysate ....................................................15.0g
Papaic digst of soybean meal........................................................5.0g
NaCl..............................................................................................5.0g
KH 2 PO 4 .........................................................................................5.0g
Glucose .........................................................................................1.0g
Esculin ..........................................................................................1.0g
Thallous acetate ............................................................................0.5g
Ferric citrate..................................................................................0.5g
Polysorbate 80.............................................................................0.75g
Bicarbonate solution................................................................10.0mL
pH 7.3 ± 0.2 at 25°C
Source: This medium is available from HiMedia.
Bicarbonate Solution:
Composition per 10.0mL:
NaHCO 3 ........................................................................................1.0g
Preparation of Bicarbonate Solution:  Add components to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components, except bicarbonate solution, to distilled/deionized water and bring volume to 990.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°C. Aseptically add bicarbonate solution. Mix thoroughly. Pour into Petri dishes or aseptically distribute into sterile tubes.
Use: For the recovery of enterococci from food.