Gum Listeria Medium (Gum Base Nalidixic Acid Medium)

[所属分类:培养基配方] [发布时间:2021-3-19] [发布人: ] [阅读次数:] [返回]

Gum Listeria Medium

(Gum Base Nalidixic Acid Medium)

培养基配方

Composition per liter:
Gellan gum....................................................................................8.0g
Casein enzymic hydrolysate.........................................................5.7g
NaCl..............................................................................................1.7g
Papaic digest of soybean meal......................................................1.0g
Dextrose......................................................................................0.83g
K 2 HPO 4 .......................................................................................0.83g
MgCl 2 ·6H 2 O ...............................................................................0.33g
Nalidixic acid..............................................................................0.05g
pH 7.2 ± 0.2 at 25°C

Source: This medium is available from HiMedia. 

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.

Use: For the isolation of Listeria monocytogenes from clinical and nonclinical specimens.