Guttman's IIB Medium

[所属分类:培养基配方] [发布时间:2021-3-19] [发布人: ] [阅读次数:] [返回]

Guttman's IIB Medium

培养基配方

Composition per liter:
Sucrose........................................................................................10.0g
Triethanolamine............................................................................5.0g
L -Glutamic acid.............................................................................1.0g
MgSO 4 ·7H 2 O................................................................................0.8g
NaCl..............................................................................................0.5g
L -Arginine.....................................................................................0.3g
L -Histidine.....................................................................................0.2g
K 3 PO 4 ............................................................................................0.2g
DL -Methionine ..............................................................................0.2g
Salts solution..............................................................................1.0mL
Vitamin solution.........................................................................1.0mL
Salts Solution:
Composition per 100.0mL:
CaCl 2 .............................................................................................0.3g
Fe(NH 4 ) 2 (SO 4 ) 2 ·6H 2 O ..................................................................0.2g
MnSO 4 ·H 2 O..................................................................................0.1g
ZnSO 4 ·7H 2 O.................................................................................0.1g
CuSO 4 ·5H 2 O...............................................................................0.04g
CoSO 4 ·7H 2 O...............................................................................0.01g
H 3 BO 3 .........................................................................................0.01g
Ammonium molybdate.............................................................0.005g
Preparation of Salts Solution: Add components to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly.
Vitamin Solution:
Composition per 100.0mL:
Calcium  D -(+)-pantothenate..........................................................0.1g
Nicotinic acid................................................................................0.1g
Pyridoxamine·HCl ........................................................................0.1g
Thiamine·HCl ...............................................................................0.1g
p-Aminobenzoic acid................................................................0.001g
Biotin ........................................................................................0.001g
Preparation of Vitamin Solution: Add components to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize. Store at 4°C.

Preparation of Medium: Add components, except vitamin solution, to distilled/deionized water and bring volume to 999.0mL. Mix thoroughly. Autoclave for 18 min at 15 psi pressure–121°C. Aseptically add 1.0mL of sterile vitamin solution. Mix thoroughly. Aseptically distribute into sterile screw-capped tubes.

Use: For the cultivation of Crithidia oncopelti.