H Broth

[所属分类:培养基配方] [发布时间:2021-3-23] [发布人: ] [阅读次数:] [返回]

H Broth

培养基配方

Composition per liter:
NaCl..............................................................................................5.0g
Pancreatic digest of casein............................................................5.0g
Peptone..........................................................................................5.0g
Beef extract...................................................................................3.0g
K 2 HPO 4 .........................................................................................2.5g
Glucose .........................................................................................1.0g
pH 7.2 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes in 4.0mL volumes. Autoclave for 15 min at 10 psi pressure–115°C. 

Use: For the preparation of the H agglutination antigen used in the differentiation and identification of Salmonella species types and subtypes.