Hagedorn and Holt Selective Medium

[所属分类:培养基配方] [发布时间:2021-3-23] [发布人: ] [阅读次数:] [返回]

Hagedorn and Holt Selective Medium

培养基配方

Composition per liter:
NaCl............................................................................................20.0g
Agar............................................................................................15.0g
Yeast extract..................................................................................2.0g
Pancreatic digest of casein............................................................1.7g
Agar..............................................................................................1.5g
NaCl..............................................................................................0.5g
Papaic digest of soybean meal......................................................0.3g
K 2 HPO 4 .......................................................................................0.25g
Glucose.......................................................................................0.25g
Cycloheximide..............................................................................0.1g
Methyl Red..............................................................................0.15mg
pH 7.3 ± 0.2 at 25°C
Caution:  Cycloheximide is toxic. Avoid skin contact or aerosol formation and inhalation.
Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.
Use: For the selective isolation of Arthrobacter species in soil.