Halobacteriaceae Medium 2

[所属分类:培养基配方] [发布时间:2021-3-25] [发布人:王亚萍] [阅读次数:] [返回]

Halobacteriaceae Medium 2

培养基配方

Composition per liter:
NaCl..........................................................................................250.0g
MgSO 4 ·7H 2 O..............................................................................20.0g
Yeast extract................................................................................10.0g
Casamino acids.............................................................................7.5g

Trisodium citrate...........................................................................3.0g

KCl................................................................................................2.0g
FeCl 2 ..........................................................................................2.3mg
pH 7.5–7.8 at 25°C
Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat until dissolved. Adjust pH to 7.5–7.8. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.
Use: For the axenic cultivation of halobacteria and halococci.