Halobacterium volcanii Medium

[所属分类:培养基配方] [发布时间:2021-3-26] [发布人: ] [阅读次数:] [返回]

Halobacterium volcanii Medium

培养基配方

Composition per liter:
NaCl..........................................................................................125.0g
MgCl 2 ·6H 2 O ...............................................................................50.0g
K 2 SO 4 ...........................................................................................5.0g
Pancreatic digest of casein............................................................5.0g
Yeast extract..................................................................................5.0g
CaCl 2 ·6H 2 O ..................................................................................0.2g
pH 6.8 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat until dissolved. Adjust pH to 6.8. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.
Use: For the cultivation of Halobacterium volcanii.