Halobacteroides Medium

[所属分类:培养基配方] [发布时间:2021-3-26] [发布人: ] [阅读次数:] [返回]

Halobacteroides Medium

培养基配方

Composition per 990.0mL:
NaCl............................................................................................88.0g
MgCl 2 ·6H 2 O ...............................................................................20.0g
CaCl 2 ·2H 2 O ..................................................................................7.4g
Yeast extract..................................................................................5.0g
KCl................................................................................................3.7g
L- Cysteine·HCl·H 2 O......................................................................0.5g
Resazurin ...................................................................................1.0mg
Glucose (10% solution) ...........................................................50.0mL
Sodium PIPES (piperazine-N,N′-bis-2-ethane
sulfonate buffer, 1M, pH 6.8–7.0).....................................40.0mL
pH 6.8–7.0 at 25°C
Preparation of Medium: Filter sterilize glucose solution and PIPES buffer solution separately. Add remaining components—except glucose solution, PIPES buffer solution, and  L -cysteine·HCl·H 2 O—to distilled/deionized water and bring volume to 900.0mL. Mix thoroughly. Gently heat and bring to boiling under 100% N 2 . Add  L -cysteine·HCl·H 2 O. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add 50.0mL of sterile glucose solution and 40.0mL of sterile PIPES buffer solution. Mix thoroughly. Aseptically and anaerobically distribute into sterile tubes or flasks.
Use:  For the cultivation and maintenance of Halobacteroides halobius and Sporohalobacter marismortui.