Halococcus Agar

[所属分类:培养基配方] [发布时间:2021-3-26] [发布人: ] [阅读次数:] [返回]

Halococcus Agar

培养基配方

Composition per liter:
Solution A..............................................................................500.0mL
Solution B..............................................................................100.0mL
Solution C..............................................................................400.0mL
pH 8.4 ± 0.2 at 25°C
Solution A:
Composition per 500.0mL:
Skim milk powder.......................................................................50.0g
Preparation of Solution A: Add skim milk powder to distilled/deionized water and bring volume to 500.0mL. Autoclave for 15 min at 8 psi pressure–112°C. Cool to 50°–55°C.
Solution B:
Composition per 100.0mL:
NaCl..........................................................................................200.0g
KNO 3 ............................................................................................2.0g
MgSO 4 ·7H 2 O.......................................................................... 10.0mg
Ferric citrate................................................................................1.0μg
Preparation of Solution B: Add components to distilled/deionized water and bring volume to 100.0mL. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°–55°C.
Solution C:
Composition per 400.0mL:
Agar ............................................................................................25.0g
Glycerol ......................................................................................10.0g

Neopeptone...................................................................................5.0g

Preparation of Solution C: Add components to distilled/deionized water and bring volume to 400.0mL. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50–55°C. Preparation of Medium: Aseptically combine 100.0mL of sterile solution B with 400.0mL of sterile solution C. Mix thoroughly. Adjust pH to 8.4. Aseptically add 500.0mL of sterile solution A. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes.
Use: For the cultivation and maintenance of Halococcus morrhuae.