Haloferax sulfurifontis Medium (DSMZ Medium 1018)

[所属分类:培养基配方] [发布时间:2021-3-26] [发布人: ] [阅读次数:] [返回]

Haloferax sulfurifontis Medium

(DSMZ Medium 1018)

培养基配方

Composition per liter:
Casitone .......................................................................................3.0g
MgSO 4 · 7H 2 O................................................................................2.0g
CaCl 2 ·2H 2 O.................................................................................0.07g
Trace elements solution SL-4 ....................................................1.0mL
Vitamin solution.........................................................................1.0mL
pH 7.1 ± 0.2 at 25°C
Vitamin Solution:
Composition per 10.0mL:
Vitamin B 12 ................................................................................5.0mg
Preparation of Vitamin Solution:  Add vitamin B 12 to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.
Trace Elements Solution SL-4:
Composition per liter:
EDTA............................................................................................0.5g
FeSO 4 ·7H 2 O................................................................................. 0.2g

Trace elements solution SL-6 ................................................100.0mL

Trace Elements Solution SL-6:
Composition per liter:
MnCl 2 ·4H 2 O .................................................................................0.5g
H 3 BO 3 ...........................................................................................0.3g
CoCl 2 ·6H 2 O..................................................................................0.2g
ZnSO 4 ·7H 2 O................................................................................ 0.1g
Na 2 MoO 4 ·2H 2 O......................................................................... 0.03g
NiCl 2 ·6H 2 O.................................................................................0.02g
CuCl 2 ·2H 2 O................................................................................0.01g
Preparation of Trace Elements Solution SL-6: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C.  Preparation of Trace Elements Solution SL-4: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C.

Preparation of Medium: Add components, except vitamin solution, to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.1. Gently heat while stirring and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to room temperature. Aseptically add 1.0mL vitamin solution. Aseptically distribute into sterile tubes or flasks.
Use: For the cultivation of Haloferax sulfurifontis.