Halomonas pantelleriensis Medium (LMG Medium 268)

[所属分类:培养基配方] [发布时间:2021-3-29] [发布人: ] [阅读次数:] [返回]

Halomonas pantelleriensis Medium

(LMG Medium 268)

培养基配方

Composition per liter:
NaCl..........................................................................................100.0g
Agar ............................................................................................20.0g
Yeast extract................................................................................10.0g
Sodium citrate...............................................................................3.0g
KCl................................................................................................2.0g
MgSO 4 ·7H 2 O................................................................................1.0g
Sodium carbonate solution....................................................20-30mL
Manganese chloride solution.....................................................1.0mL
Iron sulfate solution...................................................................1.0mL
pH 9.0 ± 0.2 at 25°C
Manganese Chloride Solution:
Composition per 100.0mL:
MnCl 2 ·4H 2 O..............................................................................0.036g
Preparation of Manganese Chloride Solution:  Add MnCl 2 ·4H 2 O to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly.
Iron Sulfate Solution:
Composition per 10.0mL:
FeSO 4 ·7H 2 O..................................................................................0.5g
Preparation of Iron Sulfate Solution:  Add FeSO 4 ·7H 2 O to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Sodium Carbonate Solution:
Composition per 100.0mL:
Na 2 CO 3 .......................................................................................10.0g
Preparation of Sodium Carbonate Solution:  Add Na 2 CO 3  to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly.
Preparation of Medium: Add components, except sodium carbonate solution, to 950.0mL distilled/deionized water. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Adjust pH to 9.0 by septically adding sterile sodium carbonate solution (20–30mL). Bring volume to 1.0L by adding sterile distilled/deionized water. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes.
Use:  For the cultivation of Halomonas pantelleriensis.