Halophile Medium

[所属分类:培养基配方] [发布时间:2021-3-29] [发布人: ] [阅读次数:] [返回]

Halophile Medium

培养基配方

Composition per liter:
NaCl..........................................................................................100.0g
KCl................................................................................................5.0g
MgCl 2 ·6H 2 O..................................................................................5.0g
MgSO 4 ·7H 2 O................................................................................5.0g
NH 4 Cl ...........................................................................................5.0g
Peptone solution (15% solution)..............................................30.0mL
Yeast extract solution (15% solution)......................................30.0mL
Ferric citrate solution (1% solution)........................................10.0mL
Trace elements solution .............................................................5.0mL
Trace Elements Solution:
Composition per liter:
ZnSO 4 ·7H 2 O...............................................................................0.22g
MgCl 2 ·4H 2 O................................................................................0.18g
CoCl 2 ·6H 2 O ................................................................................0.01g
Na 2 MoO 4 ·H 2 O ...........................................................................6.3mg
CuSO 4 ·5H 2 O..............................................................................1.0mg
Preparation of Trace Elements Solution:  Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.
Use:  For the cultivation of Rhodospirillum salinarum.