Halophilic Chromatium Medium

[所属分类:培养基配方] [发布时间:2021-3-29] [发布人: ] [阅读次数:] [返回]

Halophilic Chromatium Medium

培养基配方

Composition per 1060.0mL:
NaCl............................................................................................60.0g
KH 2 PO 4 .........................................................................................1.0g
NH 4 Cl ...........................................................................................1.0g
MgCl 2 ·6H 2 O..................................................................................0.5g
Solution A................................................................................20.0mL
Solution B................................................................................20.0mL
Solution C................................................................................10.0mL
Solution D................................................................................10.0mL
Trace elements solution .............................................................1.0mL
pH 7.0 ± 0.2 at 25°C
Solution A:
Composition per 100.0mL:
NaHCO 3 ......................................................................................10.0g
Preparation of Solution A:  Add NaHCO 3 to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C.
Solution B:
Composition per 100.0mL:
Na 2 S·9H 2 O..................................................................................10.0g
Preparation of Solution B:  Add Na 2 S·9H 2 O to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C.
Solution C:
Composition per 100.0mL:
Na 2 S 2 O 3 ·9H 2 O............................................................................10.0g
Preparation of Solution C:  Add Na 2 S 2 O 3 ·9H 2 O to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C.
Solution D:
Composition per 100.0mL:
Sodium malate............................................................................10.0g
Preparation of Solution D:  Add sodium malate to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C.
Trace Elements Solution:
Composition per liter:
FeCl 3 ·6H 2 O...................................................................................2.7g
H 3 BO 3 ...........................................................................................0.1g
ZnSO 4 ·7H 2 O.................................................................................0.1g
Co(NO 3 ) 2 ·6H 2 O.......................................................................50.0mg
CuSO 4 ·5H 2 O..............................................................................5.0mg
MnCl 2 ·6H 2 O ..............................................................................5.0mg
Preparation of Trace Elements Solution:  Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Preparation of Medium: Add components, except solution A, solution B, solution C, and solution D, to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Bring pH to 7.0–7.2 with H 3 PO 4 . Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Aseptically add 0.2mL of sterile solution A, 0.2mL of sterile solution B, 0.1mL of sterile solution C, and 0.1mL of sterile solution D for each 10.0mL of medium. Mix thoroughly. Use immediately.
Use:  For the cultivation of halophilic Chromatium species.