Halorhabdus utahensis Medium (DSMZ Medium 927)

[所属分类:培养基配方] [发布时间:2021-3-30] [发布人: ] [阅读次数:] [返回]

Halorhabdus utahensis Medium

(DSMZ Medium 927)

培养基配方

Composition per 1003.25mL:
NaCl..........................................................................................270.0g
MgSO 4 ·7H 2 O..............................................................................20.0g
Tris-HCl......................................................................................12.0g
KCl................................................................................................5.0g
NH 4 Cl ...........................................................................................2.0g
Glucose.........................................................................................2.0g
Yeast extract..................................................................................1.0g
NaBr..............................................................................................0.1g
Potassium phosphate solution....................................................2.5mL
Calcium chloride solution..........................................................0.5mL
Iron chloride manganese chloride solution..............................0.25mL
pH 7.6 ± 0.2 at 25°C
Potassium Phosphate Solution:
Composition per 10.0mL:
KH 2 PO 4 .........................................................................................0.5g

Preparation of Potassium Phosphate Solution: Add KH 2 PO 4 to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to room temperature.

Calcium Chloride Solution:
Composition per 10.0mL:
CaCl 2 ·2H 2 O...................................................................................1.0g
Preparation of Calcium Chloride Solution: Add CaCl 2 ·2H 2 O to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to room temperature.
Iron Chloride Manganese Chloride Solution:
Composition per 10.0mL:
FeCl 2 ·4H 2 O...................................................................................0.2g
MnCl 2 ·4H 2 O..................................................................................0.2g
Preparation of Iron Chloride Manganese Chloride Solution: Add components to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to room temperature.
Preparation of Medium: Add components, except potassium phosphate solution, calcium chloride solution, and iron chloride manganese chloride solution, to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.6 with 5M NaOH. Autoclave for 15 min at 15 psi pressure–121°C. Cool to room temperature. Aseptically add 2.5mL phosphate solution, 0.5mL calcium chloride solution, and 0.25mL iron chloride manganese chloride solution. Mix thoroughly. Aseptically distribute to sterile tubes or flasks.
Use: For the cultivation of Halorhabdus utahensis.