Halorubrum californiense Medium (DSMZ Medium 1194)

[所属分类:培养基配方] [发布时间:2021-3-30] [发布人: ] [阅读次数:] [返回]

Halorubrum californiense Medium

(DSMZ Medium 1194)

培养基配方

Composition per liter:
NaCl..........................................................................................156.0g
MgSO 4 · 7H 2 O..............................................................................40.6g
MgCl 2 ·6H 2 O................................................................................26.0g
Yesat extract................................................................................10.0g
KCl................................................................................................4.0g
CaCl 2 ·2H 2 O.................................................................................0.66g
NaBr............................................................................................0.46g
NaHCO 3 ......................................................................................0.12g
pH 7.2 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.2. Gently heat while stirring and bring to boiling. Mix thoroughly. Distribute into tubes , bottles, or flasks. Autoclave for 15 min at 15 psi pressure–121°C.
Use: For the cultivation of Halorubrum californiense.