HC Agar Base

[所属分类:培养基配方] [发布时间:2021-3-31] [发布人: ] [阅读次数:] [返回]

HC Agar Base

培养基配方

Composition per liter:
Glucose.......................................................................................20.0g
Agar............................................................................................15.0g
Yeast extract..................................................................................5.0g
Na 2 HPO 4 .......................................................................................3.5g
KH 2 PO 4 .........................................................................................3.4g
Pancreatic digest of casein............................................................2.5g
Peptic digest of animal tissue .......................................................2.5g
NH 4 Cl ...........................................................................................1.4g
Na 2 CO 3 .........................................................................................1.0g
Chloramphenicol...........................................................................0.1g
MgSO 4 ·7H 2 O..............................................................................0.06g
Polysorbate 80 (Tween™ 80) solution ....................................20.0mL
pH 7.0 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from BD Diagnostic Systems.
Preparation of Medium: Add components, except Tween™ 80, to distilled/deionized water and bring volume to 980.0mL. Mix thoroughly. Gently heat and bring to boiling. Add Tween™ 80. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes.
Use:  For the cultivation and enumeration of molds in cosmetics and toiletries.